Artificial feeding of Ornithodoros fonsecai (Acari: Argasidae) with the anticoagulant Alsever

Ana C. Castro-Santiago; Simone M. Simons; Leidiane Lima-Duarte; Jaqueline V. Camargo; Rosangela Z. Machado; Marco R. André; Darci M. Barros-Battesti

doi:10.37486/2675-1305.ec03047

Authors

Ana C. Castro-Santiago Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, SP https://orcid.org/0000-0002-2660-0958
Simone M. Simons Parasitology Laboratory, Instituto Butantan, São Paulo, SP https://orcid.org/0000-0003-3390-4535
Leidiane Lima-Duarte Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, SP
Jaqueline V. Camargo Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, UNESP, Jaboticabal, SP https://orcid.org/0000-0003-3147-5650
Rosangela Z. Machado Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, UNESP, Jaboticabal, SP https://orcid.org/0000-0002-5176-2940
Marco R. André Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, UNESP, Jaboticabal, SP https://orcid.org/0000-0002-1713-5222
Darci M. Barros-Battesti Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo/Department of Pathology, Reproduction and One Health, Faculty of Agrarian and Veterinary Sciences, UNESP, Jaboticabal, SP https://orcid.org/0000-0002-8541-2252

DOI:

https://doi.org/10.37486/2675-1305.ec03047

Keywords:

argasid tick, artificial feeding, anticoagulant, biology

Abstract

Ornithodoros fonsecai is a species of argasid tick endemic to Brazil, described in the “São Miguel” cave located in the municipality of Bonito, state of Mato Grosso do Sul, central-western region of Brazil. The artificial feeding technique makes it possible to study the biology of hematophagous arthropods using artificial or natural membranes, as well as different types of blood and anticoagulants. Thus, the aim of the present study was to feed artificially O. fonsecai second instar (N2) nymphs with rabbit blood using parafilm membrane and the anticoagulant Alsever. Ninety percent of the total N2 nymphs engorged and molted to N3 nymphs between 27 and 30 days after feeding, indicating that the use of this anticoagulant is efficient for artificially feeding O. fonsecai N2 nymphs under laboratory conditions.

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