Terapia larval: protocolo básico de manutenção, desinfecção, transporte e aplicação de larvas de Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae)

Renata A. Gama; Paula B. T. Brambilla; Sarah R. A. Silva; Jucélia R. Medeiros; Jéssica T. Jales; Marília A. R. Q. Pinheiro; Julianny B. Ferraz; Renato Motta Neto; Taciano M. Barbosa

doi:10.37486/2675-1305.ec03022

Authors

Renata A. Gama Laboratório de Insetos e Vetores-LIVe, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0002-8026-6022
Paula B. T. Brambilla Laboratório de Insetos e Vetores-LIVe, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0002-8009-4582
Sarah R. A. Silva Laboratório de Insetos e Vetores-LIVe, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0002-8806-4855
Jucélia R. Medeiros Laboratório de Insetos e Vetores-LIVe, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0003-4982-9365
Jéssica T. Jales Laboratório de Insetos e Vetores-LIVe, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0002-8502-679X
Marília A. R. Q. Pinheiro Laboratório de Insetos e Vetores-LIVe, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal/Hospital Universitário Onofre Lopes. Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0003-4867-6694
Julianny B. Ferraz Hospital Universitário Onofre Lopes. Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0003-4305-0959
Renato Motta Neto Laboratório de Micobactérias. Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0002-6112-7801
Taciano M. Barbosa Laboratório de Insetos e Vetores-LIVe, Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal https://orcid.org/0000-0002-5495-258X

DOI:

https://doi.org/10.37486/2675-1305.ec03022

Keywords:

Blowflies, larval therapy, rearing protocol, biotherapy

Abstract

Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) is an exotic blowfly in Brazil and it is considered one of the main species of medical-forensic importance. Their larvae have a necrophagous habit with a high forensic potential and they are also considered an important tool for treating chronic wounds, a technique known as maggot therapy, which is not yet routinely used in Brazil despite proven efficacy. In this context, the present work aims to describe a protocol for carrying out the technique, with the complete description of colony maintenance, preparation and disinfection of eggs, packing and transportation of larvae for use, application and removal of the larvae on patients. Thus, with this protocol, the maggot therapy can be disseminated and performed by other research groups.

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References

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