Molecular confirmation of the presence of the South American tomato leafminer, Phthorimaea absoluta Meyrick, 1917 (Lepidoptera: Gelechiidae) in Lao PDR


  • Viengvilay Vorlachith Provincial Agriculture and Forestry Office, Pakse, Champasak, Lao PDR
  • Sengphet Phanthavong Provincial Agriculture and Forestry Office, Pakse, Champasak, Lao PDR
  • Somkhit Sengsay Ministry of Agriculture and Forestry, Vientiane, Lao PDR
  • Salah Hafidi Sevea Consulting, Khan Chamkarmorn, Phnom Penh, Cambodia
  • Hang Thi Dao Plant Protection Research Institute, Ha Noi, Viet Nam
  • Chanthason Vongtharone Provincial Agriculture and Forestry Office, Pakse, Champasak, Lao PDR
  • Bouasa Xaisongkham Provincial Agriculture and Forestry Office, Pakse, Champasak, Lao PDR
  • Kitan Sitthisay Provincial Agriculture and Forestry Office, Pakse, Champasak, Lao PDR
  • George A. C. Beattie Western Sydney University, Penrith, NSW, Australia
  • Paul Holford Western Sydney University, Penrith, NSW, Australia
  • Tara Garrard South Australian Research and Development Institute, Urrbrae, SA, Australia
  • Sally Cowan Northern Australian Quarantine Strategy, Cairns, QLD, Australia
  • Lester W. Burgess University of Sydney, Sydney, NSW, Australia



Tuta absoluta; Morphology, Molecular identification, Tomato, Eggplant


Molecular confirmation of the presence of the tomato leafminer, Phthorimaea (Tuta) absoluta Meyrick, 1917 (Lepidoptera: Gelechiidae), in the Paksong region of the Bolaven Plateau in Champasak province in south-western Lao PDR is reported. The moth, which is native to South America, has invaded many countries in Europe, Africa, and Asia since 2006, and, based on regional records possibly entered Lao PDR between 2014 and 2018. It is a devastating pest of solanaceous crops and threatens tomato production worldwide. Further surveys are required to determine its presence in other regions of Lao PDR and effective management programs must be developed to help farmers minimise losses.


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Berxolli, A.; Shahini, S. (2017) Controlling Tuta absoluta (Meyrick, 1917) based on emamectin benzoate usage. European Journal of Physical and Agricultural Sciences. 6: 1-6.

Brambila, J.; Lee, S.; Passoa, S. (2010) Tuta absoluta. The tomato leafminer. Field Screening Aid. Cooperative Agriculture Pest Survey program.

Calvo, F. Z.; Lorente, M. J.; Stansly, P. A.; Belda J. E. (2012) Preplant release of Nesidiocoris tenuis and supplementary tactics for control of Tuta absoluta and Bemisa tabaci in greenhouse tomato. Entomologia Experimentalis et Applicata 143: 111-119. DOI: 10.1111/j.1570-7458.2012.01238.x

Dao, H. T.; Beattie, G. A. C.; Watson, G. W.; Mottern, J.; Evans, G.; Nguyen, L. V.; Nguyen, H. T.; Nguyen, V. D.; Holford, P. (2020) Discovery of false coconut scale (Aspidiotus rigidus) and three of its primary parasitoids in Việt Nam, and likely species origins. Journal of Asia-Pacific Entomology, 35(2): 395-403. DOI: 10.1016/j.aspen.2020.02.002

Delang, C. O.; Toro, M.; Charlet-Phommachanh, M. (2013) Coffee, mines and dams: conflicts over land in the Bolaven Plateau, southern Lao PDR. The Geographical Journal 179(2): 150-64. DOI: 10.1111/j.1475-4959.2012.00481.x

Desneux, N.; Han, P.; Mansour, R.; Arnó, J.; Brévault, T.; Campos, M.; Chailleux, A.; Guedes, R. N. C.; Karimi, J.; Konan, K. A. J., et al. (2022) Integrated pest management of Tuta absoluta: practical implementations across different world regions. Journal of Pest Science, 95(1): 17-39. DOI: 10.1007/s10340-021-01442-8

Desneux, N.; Luna, M. G.; Guillemaud, T. T.; Urbaneja, A. (2011) The invasive South American tomato pinworm, Tuta absoluta, continues to spread in Afro-Eurasia and beyond: the new threat to tomato world production. Journal of Pest Science, 84(4): 403-08. DOI: 10.1007/s10340-011-0398-6

Desneux, N.; Wajnberg, E.; Wyckhuys, K. A. G.; Burgio, G.; Arpaia, S.; Narváez-Vasquez, C. A.; González-Cabrera, J.; Ruescas, D. C.; Tabone, E.; Frandon, J., et al. (2010) Biological invasion of European tomato crops by Tuta absoluta: ecology, geographic expansion and prospects for biological control. Journal of Pesticide Science, 83(3): 197-215. DOI: 10.1007/s10340-010-0321-6

Folmer, O.; Black, M.; Hoeh, W.; Lutz, R.; Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294-99.

Guedes, R.; Roditakis, E.; M. Campos, M.; Haddi, K.; Bielza, P.; Siqueira, H.; Tsagkarakou, A.; Vontas, J.; Nauen, R. (2019) Insecticide resistance in the tomato pinworm Tuta absoluta: patterns, spread, mechanisms, management and outlook. Journal of Pest Science, 92 (4): 1329-1342. DOI: 10.1007/s10340-019-01086-9

Guillemaud, T.; Blin, A.; Le Goff, I.; Desneux, N.; Reyes, M.; Tabone, E.; Tsagkarakou, A.; Niño L.; Lombaert, E. (2015) The tomato borer, Tuta absoluta, invading the Mediterranean Basin, originates from a single introduction from Central Chile. Science Reports, 5(1): 8371. DOI: 10.1038/srep08371

Guimapi, R. A.; Srinivasan, R.; Tonnang, H. E.; Sotelo-Cardona, P.; Mohamed, S. A. (2020) Exploring the mechanisms of the spatiotemporal invasion of Tuta absoluta in Asia. Agriculture, 10(4): 124. DOI 10.3390/agriculture10040124

Kılıç, T. (2009) First record of Tuta absoluta in Turkey. Phytoparasitica, 38(3): 243-44. DOI: 10.1007/s12600-010-0095-7

Mahlangu, L.; Sibisi, P.; Nofemela, R. S.; Ngmenzuma, T.; Ntushelo, K. (2022) The differential effects of Tuta absoluta infestations on the physiological processes and growth of tomato, potato, and eggplant. Insects, 13(8): 754. DOI: 10.3390/insects13080754

Maluf, W. R.; Silva V. F.; Cardoso, M. G.; Gomes, L. A. A.; Gonçalves Neto, A. C.; Maciel, G. M., Nízio, D. A. C. (2010) Resistance to the South American tomato pinworm Tuta absoluta in high acylsugar and/or high zingiberene tomato genotypes. Euphytica, 176(1): 113-123. DOI: 10.1007/s10681-010-0234-8

Mutamiswa, R.; Machekano, H.; Nyamukondiwa, C. (2017) First report of tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), in Botswana. Agriculture & Food Security, 6(1): 1-10. DOI: 10.1186/s40066-017-0128-2

Nguyễn, V. L.; Đào, T. H. (2019) Lần đầu ghi nhận sâu vẽ bùa nam mỹ Tuta absoluta (Lepidoptera: Gelechiidae) ở Việt Nam: Hình thái, cơ quan sinh dục và phân tử (First record of South American tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae) in Viet Nam: Morphology, genitalia and molecular.). Plant Protection Journal, 4: 3-9.

Rondon S. I; Gao Y. (2022) Potato Tuberworm. In: Alyokhin A.; Rondon S. I.; Gao Y. (Eds.). Insect pest of potatoes: Global Perspectives on Biology and Management, Second Edition, pp 149-166. Academic Press.

Rwomushana, I.; Beale, T.; Chipabika, G.; Day, R.; Gonzalez-Moreno, P.; Lamontagne-Godwin, J.; Makale, F.; Pratt, C.; Tambo, J. (2019) Evidence note. Tomato leafminer (Tuta absoluta): impacts and coping strategies for Africa. CABI Working Paper 12, DOI: 10.1079/CABICOMM-62-8100

Soares, M. A.; Campos, M. R.; Passos, L. C.; Carvalho, G. A.; Haro, M. M.; Lavoir, A. V.; Biondi, A.; Zappalà. L.; Desneux, N. (2019) Botanical insecticide and natural enemies: a potential combination for pest management against Tuta absoluta. Journal of Pest Science, 92(4): 1433-1443. DOI: 10.1007/s10340-018-01074-5

Sridhar, V.; Chakravarthy, A. K.; Asokan, R.; Vinesh, L. S.; Rebijith., K. B.; Vennila, S. (2014) New record of the invasive South American tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in India. Pest Management in Horticultural Ecosystems, 20: 148-54.

Tamura, K.; Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10: 512-26. DOI: 10.1093/oxfordjournals.molbev.a040023

Toševski, I.; Jović J.; Mitrović, M.; Cvrković, T.; Krstić, O.; Krnjajić, S. (2011) Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae): a new pest of tomato in Serbia. Journal Pesticides and Phytomedicine, 26(3): 197-204. DOI: 10.2298/PIF1103197T

Tropea Garzia, G.; Siscaro, G.; Biondi, A.; Zappalà, L. (2012) Tuta absoluta, a South American pest of tomato now in the EPPO region: biology, distribution and damage. EPPO Bulletin, 42(2): 205-10. DOI: 10.1111/epp.2556

Yule, S.; Htain, N. N.; Oo, A. K.; Sotelo-Cardona, P.; Srinivasan, R. (2021) Occurrence of the South American tomato leafminer, Tuta absoluta (Meyrick) in Southern Shan, Myanmar. Insects, 12(11): 962. DOI: 10.3390/insects12110962

Zhang, G. F.; Ma, D. Y.; Wang, Y. S.; Gao, Y. H.; Liu, W. X.; Zhang, R.; Fu, W.J.; Xian, X. Q.; Wang, J.; Kuang, M.; Wan, F. H. (2020) First report of the South American tomato leafminer, Tuta absoluta (Meyrick), in China. Journal of Integrative Agriculture, 19(7): 1912-17. DOI: 10.1016/S2095-3119(20)63165-3




How to Cite

Vorlachith, V., Phanthavong, S., Sengsay, S., Hafidi, S., Dao, H. T., Vongtharone, C., … Burgess, L. W. (2024). Molecular confirmation of the presence of the South American tomato leafminer, Phthorimaea absoluta Meyrick, 1917 (Lepidoptera: Gelechiidae) in Lao PDR. Entomological Communications, 6, ec06005.



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