Efeitos não-alvo de inseticidas utilizados para controle de Bemisia tabaci (Gennadius, 1889) biótipo B (Hemiptera: Aleyrodidae) sobre a mortalidade e biologia de Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) em soja [Glycine max (l.) Merrill]





fall armyworm, lufenuron, buprofezin, pyriproxyfen, diafenthiuron, mortality


Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) is a polyphagous pest that has gained importance in soybean cultivation in recent years, due to the damage it causes to leaves and pods. The objective of this work was to evaluate the non-target effect of insecticides recommended and used to control Bemisia tabaci (Genn.) biotype B on S. frugiperda. The experimental design was entirely randomized with five treatments and fifty replications. The treatments were: 1. negative control (distilled water), 2. positive control (lufenuron), 3. buprofezin, 4. pyriproxyfen, and 5. diafenthiuron. In each replicate, one caterpillar was placed in a plastic cup, where sprayed soybean leaves with the maximum dosage of insecticides recommended for B. tabaci were placed.The treatments were evaluated daily during all stages: larval, pupal, and adult emergence and fecundity. The results obtained for larval mortality showed that diafenthiuron aimed 100% control, followed by pyriproxyfen with 28% and buprofezin with 12%. The duration of the larval period of the insects exposed to pyriproxyfen and buprofezin were longer compared to the control. While the biological parameters of pupae were found that pyriproxyfen caused lower pupal formation rate and higher pupal mortality, followed by buprofezin. In adult emergence, pyriproxyfen showed the lowest rate with 13.8% and the highest percentage of deformed adults with 60%. Thus, it was possible to conclude that the insecticides under study cause S. frugiperda mortality or effects on its biological patterns.


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Abbott, W. S. (1925) A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267. doi: 10.1093/jee/18.2.265a

Adel, M. M. (2012) Lufenuron impair the chitin synthesis and development of Spodoptera littoralis Bosid. (Lepidoptera: Noctuidae). Journal of Applied Sciences Research, 8(5): 27-66.

Amaral, F. S.; Guidolin, A. S.; Salmeron, E.; Kanno, R. H.; Padovez, F. E.; Fatoretto, J. C.; Omoto, C. (2020) Geographical distribution of Vip3Aa20 resistance allele frequencies in Spodoptera frugiperda (Lepidoptera: Noctuidae) populations in Brazil. Pest Management Science, 76(1): 169-178. doi: 10.1002/ps.5490

Bernardi, D.; Salmeron, E.; Horikoshi, R. J.; Bernardi, O.; Dourado, P. M.; Carvalho, R. A.; Martinelli, S.; Head, G. P.; Omoto, C. (2015) Cross-resistance between Cry1 proteins in fall armyworm (Spodoptera frugiperda) may affect the durability of current pyramided Bt maize hybrids in Brazil. PloS one, 10: e0140130. doi: 10.1371/journal.pone.0140130

Butt, B.; Cantu, E. (1962) Sex determination of lepidopterous pupae. USDA, Agricultural Research Service, 33(75), 1-7.

Degrande, P. E.; Vivan, L. (2012) Pragas da soja. Tecnologia e produção: soja e milho, 8: 155-206.

El-Sheikh, E. S. A.; Kamita, S. G.; Hammock, B. D. (2016) Effects of juvenile hormone (JH) analog insecticides on larval development and JH esterase activity in two spodopterans. Pesticide Biochemistry and Physiology, 128: 30-36. doi: 10.1016/j.pestbp.2015.10.008

Faria, G. (2014) Produtores devem fazer o controle da mosca-branca para evitar prejuízos na safrinha. Embrapa. https://www.embrapa.br/busca-de-noticias/-/noticia/1718061/produtores-devem-fazer-o-controle-da-mosca-branca-para-evitar-prejuizos-na-safrinha. Acesso em: 12.iv.2020.

Farias, J. R.; Andow, D. A.; Horikoshi, R. J.; Sorgatto, R. J.; Fresia, P.; dos Santos, A. C.; Omoto, C. (2014) Field-evolved resistance to Cry1F maize by Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil. Crop Protection, 64: 150-158. doi: 10.1016/j.cropro.2014.06.019

Fernandes, F. O.; Abreu, J. A.; Christi, L. M.; Rodrigues-Filho, J. A.; Martins, A.; Rosa, A. P. S. A. (2017) Desenvolvimento de Spodoptera frugiperda (Smith, 1797) em cultivares de milho em laboratório. Boletim de Pesquisa e Desenvolvimento, 264. Pelotas: Embrapa Clima Temperado

Freitas, A. F.; Formagio, A. S.; Pereira, F. F.; Lucchetta, J. T.; Vieira, M. D. C. (2014) Effect of extracts of Trichilia silvatica C. DC., on development and reproduction parameters of Spodoptera frugiperda (J.E Smith) (Lepidoptera: Noctuidae). African Journal of Biotechnology, 13(20): 2041-2049. doi: 10.5897/ajb2014.13768

Gallo, D.; Nakano, O.; Neto, S. S.; Carvalho, R. P. L.; Baptista, G. C.; Berti Filho, E.; Parra, J. R. P.; Zucchi, R. A.; Alves, S. B.; Vendramim, J. D., et al. (2002) Entomologia Agrícola. Piracicaba: Editora Fealq.

Greene, G. L.; Leppla, N. C.; Dickerson, W. A. (1976) Velvetbean caterpillar: a rearing procedure and artificial medium. Journal of Economic Entomology, 69(4): 487-488. doi: 10.1093/jee/69.4.487

Hoffman-Campo, C. B.; Oliveira, L. J.; Moscardi, F.; Corrêa-Ferreira, B. S.; Corso, I. C. (2012) Pragas que atacam plântulas, hastes e pecíolos da soja. In: Hoffmann-Campo, C. B.; Corrêa-Ferreira, B. S.; Moscardi, F. (Eds.), Soja: manejo integrado de insetos e outros Artrópodes-praga, pp. 145-199. Brasília, DF, Embrapa.

Huang, F.; Qureshi, J. A.; Meagher Jr, R. L.; Reisig, D. D.; Head, G. P.; Andow, D. A.; Xinzi, N.; Kerns, D.; Buntin, G. D., Niu, Y., et al. (2014) Cry1F resistance in fall armyworm Spodoptera frugiperda: single gene versus pyramided Bt maize. PloS One, 9(11): e112958. doi: 10.1371/journal.pone.0112958

IRAC (Insecticide Resistance Action Committee) (2018) Manejo da resistência a inseticidas e plantas Bt (Spodoptera frugiperda, Helicoverpa armígera, Chrysodeixis includens). Folheto.

Khan, M. A. M. (2016) Efficacy of insect growth regulator Buprofezin against Papaya mealybug. JEZS, 4(4), 730-733.

Khatun, M. R.; Das, G.; Ahmed, K. S.; Kato-Noguchi, H. (2020) Inhibitory effect of buprofezin on the larval growth and development of Spodoptera litura (F.). Indian Journal of Entomology, 82(1): 20-23. doi: 10.5958/0974-8172.2020.00004.8

Khorshidi, M.; Abad, R. F. P.; Saber, M.; Zibaee, A. (2019) Effects of hexaflumuron, lufenuron and chlorfluazuron on certain biological and physiological parameters of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). Biocatalysis and Agricultural Biotechnology, 21: 101270. doi: 10.1016/j.bcab.2019.101270

McCord Jr, E.; Yu, S. J. (1987) The mechanisms of carbaryl resistance in the fall armyworm, Spodoptera frugiperda (JE Smith). Pesticide Biochemistry and Physiology, 27(1): 114-122. doi: 10.1016/0048-3575(87)90103-9

Nasr, H. M.; Badawy, M. E.; Rabea, E. I. (2010) Toxicity and biochemical study of two insect growth regulators, buprofezin and pyriproxyfen, on cotton leafworm Spodoptera littoralis. Pesticide Biochemistry and Physiology, 98(2): 198-205. doi: 10.1016/j.pestbp.2010.06.007

Ono, É. K.; Zanardi, O. Z.; Santos, K. F. A.; Yamamoto, P. T. (2017) Susceptibility of Ceraeochrysa cubana larvae and adults to six insect growth-regulator insecticides. Chemosphere, 168: 49-57. doi: 10.1016/j.chemosphere.2016.10.061

Omoto, C.; Bernardi, O.; Salmeron, E.; Sorgatto, R. J.; Dourado, P. M.; Crivellari, A.; Carvalho, A.; Willse, A.; Martinelli, S.; Head, G. P. (2016) Field-evolved resistance to Cry1Ab maize by Spodoptera frugiperda in Brazil. Pest Management Science, 72(9): 1727-1736. doi: 10.1002/ps.4201

Panizzi, A. R.; Bueno, A. D. F.; Silva, F. D. (2012) Insetos que atacam vagens e grãos. Soja: manejo integrado de insetos e outros artrópodes-praga. Brasília: Embrapa.

Resende, D. C.; Mendes, S. M.; Marucci, R. C.; Silva, A. D. C.; Campanha, M. M.; Waquil, J. M. (2016) Does Bt maize cultivation affect the non-target insect community in the agro ecosystem?. Revista Brasileira de Entomologia, 60(1): 82-93. doi: 10.1016/j.rbe.2015.12.001

Riley, D.; Smith, H.; Bennett, J.; Torrance, P.; Huffman, E.; Sparks, A.; Gruver, C.; Dunn, T.; Champagne, D. (2020) Regional survey of diamondback moth (Lepidoptera: Plutellidae) response to maximum dosages of insecticides in Georgia and Florida. Journal of Economic Entomology, 113(5): 2458-2464. doi: 10.1093/jee/toaa125

Rosa, A. P. S. A.; Teixeira, H. B.; Medina, L. B.; Hellwig, L.; Fipke, M. V. (2014) Ponte Verde para Spodoptera frugiperda (JE Smith, 1797) (Lepidoptera: Noctuidae) em Terras Baixas. Embrapa Clima Temperado-Comunicado Técnico (INFOTECA-E).

Rosa, A. P. S. A.; Martins, J. D. S. (2011) Manejo da resistência de Spodoptera frugiperda a inseticidas na cultura do milho: situação atual. Embrapa Clima Temperado-Documentos (INFOTECA-E).

Ruder, F. J.; Kayser, H. A. R. T. M. U. T. (1993) The carbodiimide product of diafenthiuron inhibits mitochondria in vivo. Pesticide Biochemistry and Physiology, 46(2): 96-106. doi: 10.1006/pest.1993.1041

Sarkar, P. K. (2016) Bio-Effectiveness of a Pro-Insecticide, Diafenthiuron 50% WP Against Diamond Back Moth, Plutella xylostella (Lin.) in Cabbage in Gangetic Alluvial Plains of West Bengal. International Journal of Agriculture, Environment and Biotechnology, 9(6): 1089-1095. doi: 10.5958/2230-732x.2016.00137.6

Tavares, W. S.; Costa, M. A.; Cruz, I.; Silveira, R. D.; Serrao, J. E.; Zanuncio, J. C. (2010) Selective effects of natural and synthetic insecticides on mortality of Spodoptera frugiperda (Lepidoptera: Noctuidae) and its predator Eriopis connexa (Coleoptera: Coccinellidae). Journal of Environmental Science and Health, Part B, 45(6): 557-561. doi: 10.1080/03601234.2010.493493

Tunaz, H.; Uygun, N. (2004) Insect growth regulators for insect pest control. Turkish Journal of Agriculture and Forestry, 28(6): 377-387.

Yang, F.; Wang, Z.; Kerns, D. L. (2022) Resistance of Spodoptera frugiperda to Cry1, Cry2, and Vip3Aa proteins in Bt corn and cotton in the Americas: Implications for the rest of the world. Journal of Economic Entomology, 115(6): 1752-1760. doi: 10.1093/jee/toac099




How to Cite

Santana, L. S., Vilhasanti, C. B., Degrande, P. E., Souza, E. P., Scoton, A. M. N., & Pereira, M. D. C. (2023). Efeitos não-alvo de inseticidas utilizados para controle de Bemisia tabaci (Gennadius, 1889) biótipo B (Hemiptera: Aleyrodidae) sobre a mortalidade e biologia de Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) em soja [Glycine max (l.) Merrill]. Entomological Communications, 5, ec05038. https://doi.org/10.37486/2675-1305.ec05038